{"id":14980,"date":"2024-11-11T15:19:29","date_gmt":"2024-11-11T20:19:29","guid":{"rendered":"https:\/\/mote.org\/?page_id=14980"},"modified":"2025-11-13T15:13:01","modified_gmt":"2025-11-13T20:13:01","slug":"our-methods","status":"publish","type":"page","link":"https:\/\/mote.org\/research\/coral-biorepository-alliance-v2\/our-methods\/","title":{"rendered":"Coral Biorepostory Alliance – Our Methods"},"content":{"rendered":"

<\/i>\u00a0 Return to Coral Biorepository Alliance Home<\/a><\/small><\/p>\n

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Coral Biobank Alliance members are working collaboratively to share and standardize methods used to preserve coral genetic diversity for conservation and restoration. Currently, the methods used by our members include:<\/p>\n

Colony identification<\/h2>\n

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Coral collection from reefs (with appropriate government permits)<\/h2>\n

Microbiome samples<\/h5>\n
GPS<\/h5>\n
Tagging<\/h5>\n
Photographing colony<\/h5>\n
Measurements<\/h5>\n
Ensuring there is enough biomass for holding and sampling<\/h5>\n

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Transportation<\/h2>\n

Long distance shipping of coral colonies<\/h5>\n

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Quarantine<\/h2>\n

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Coral health and growth monitoring<\/h2>\n

Health checks<\/h5>\n
Water quality and parameters<\/h5>\n

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Live biorepository maintenance and husbandry<\/h2>\n

Coral colony maintenance in breeding systems, including in remote areas<\/h5>\n
Ex-situ coral conservation through preserving living coral biodiversity in state-of-the-art ex situ “coral ark” facilities<\/span><\/h5>\n
Microfragmentation<\/h5>\n
Vet care<\/h5>\n
Targeting resilient broodstock: Integrating corals that are more resistant to known stressors than other genotypes in addition to facilitating and promoting genetic diversity<\/span><\/h5>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

Sampling<\/h2>\n

Genomics<\/h5>\n
Genetics: Metatranscriptomics, metagenomics,16S (microbiome), genetic management planning<\/h5>\n

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Database management<\/h2>\n

 <\/p>\n

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Cryopreservation<\/h2>\n

Cryopreservation of coral sperm so they can be used later for breeding or genetic studies<\/span><\/h5>\n
Ex situ reproduction with sperm<\/h5>\n
Long term banking<\/h5>\n

 <\/p>\n

 <\/p>\n

Breeding<\/span><\/h2>\n
Collecting gametes from broodstock and combining them in different genetic crosses with the goal of fostering species resilience with hundreds to thousands of diverse offspring<\/h5>\n

<\/h2>\n

\"AGF<\/h2>\n

<\/h2>\n

Outpla<\/span>nting<\/span><\/h2>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0 Return to Coral Biorepository Alliance Home Coral Biobank Alliance members are working collaboratively to share and standardize methods used to preserve coral genetic diversity for conservation and restoration. Currently, …<\/span><\/p>\n","protected":false},"author":3,"featured_media":14993,"parent":21999,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-14980","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nCoral Biorepostory Alliance - Our Methods | Mote Marine Laboratory & Aquarium<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mote.org\/research\/coral-biorepository-alliance-v2\/our-methods\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Coral Biorepostory Alliance - Our Methods | Mote Marine Laboratory & Aquarium\" \/>\n<meta property=\"og:description\" content=\"\u00a0 Return to Coral Biorepository Alliance Home Coral Biobank Alliance members are working collaboratively to share and standardize methods used to preserve coral genetic diversity for conservation and restoration. 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